Centrifuge

Stainless steel rotor chamber is rust-proof and easy to clean. The At set rpm function starts the timer when the selected speed has been reached. There is an electronic imbalance detection feature for maximum safety.

The Executive Series Centrifuge II (ESC II) has the most advanced technology setting it apart from all predecessors. With a new elegant design, the ESC II is crafted to perfection and universality while maintaining its affordable cost. Redesigned lighter buckets enhances safety and optimizes the separation process during centrifugation. Increased power reduces overall time for processing by 2 minutes and a more smooth optimal breaking system for a more concentrated sample. Performance outcomes beats the competition. Mobility and usage is optimized with smaller, lighter, quieter, and improved engineering! Durable to last and universally designed so you can use for various clinical purposes.

Many applications in clinical and cytological laboratories require rapid processing of the samples under examination. The centrifuges required for such applications should therefore be able to accelerate as fast as possible and to brake at a correspondingly fast rate so that temperature-sensitive samples such as culture cells can be used for further steps of the relevant application without remaining in the centrifuge for a long time. With the development of the Centrifuge models 5702, 5702 R and 5702 RH, Eppendorf offers modern laboratory centrifuges for such applications that have extremely short acceleration and braking times (< 25 sec). Besides speed, another decisive criterion for the quality of a centrifuge in the routine lab is a feature called the re-mixing rate.Therefore, special attention has been paid to the development of a second acceleration and braking ramp that is gentle and yet fast.

This function is called a Soft function and enables the user to perform sensitive applications such as density gradient centrifugations to isolate blood cells quickly and reproducibly. The isolation of human mononuclear cells (MNC) from whole blood is one of these standard methods used in cytological laboratories. This cell population, which contains T- and B-lymphocytes and monocytes, can then be used directly for experiments or serves as a starting population for further isolation of single types of cells.

To isolate MNC by density gradient centrifugation, the main method used is a method based on the density properties of Ficoll (1). Due to their high density, red blood cells (erythrocytes) pass the Ficoll phase (density: 1.077 g/ml) and form a sediment. A high-polymer sugar, Ficoll also causes agglutination (clumping) of the erythrocytes, thus accelerating sedimentation. Lympohocytes, thrombocytes and monocytes collect at the plasma gradient phase (specific weight less than 1.077 g/ml) and can thus be enriched. The results of the software optimization for the development of an optimum Soft function are summarized below.

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